Protein Simple Wes Western Blot System

Protein Simple Wes Western Blot System

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Used

Model Year:

Status:

Sold

Warranty:

30 Days (TKG Promise)

1 in stock

The ProteinSimple WES system is simple to operate, by loading samples and reagents on the assay plate and the system handles the rest. This automated western blot takes over sample loading, size-based protein separation, immunoprobing or total protein labeling, washing, detection, and data analysis all within the chamber and uses just two pieces of plastic instead of the staggering 300 of traditional instruments. Users can get high throughput from as little sample as 40nL, and this helps eliminate variability.

The ProteinSimple WES Western Blot System is an automated and streamlined solution for performing western blotting assays with ease and efficiency. This innovative system simplifies the western blot workflow, from sample preparation to data analysis, enabling researchers to obtain reliable and reproducible results in a fraction of the time compared to traditional methods.

  1. Automated Sample Processing: The WES system automates the entire western blotting process, including protein separation, immunoprobing, and detection, minimizing hands-on time and reducing variability between experiments.
  2. Micro-scale Assays: With microfluidic capillary electrophoresis technology, the system enables precise separation and quantification of proteins in minute sample volumes, conserving precious samples and reagents.
  3. Rapid Turnaround Time: The WES system significantly reduces assay time, allowing for same-day results without the need for overnight incubations, accelerating research progress and data generation.
  4. High Sensitivity and Dynamic Range: The system offers exceptional sensitivity and a wide dynamic range, enabling the detection of low-abundance proteins and accurate quantification over a broad concentration range.
  5. Quantitative Analysis: Advanced software algorithms provide quantitative analysis of protein expression levels, allowing for precise comparison between samples and accurate determination of fold changes.
  6. User-friendly Interface: The intuitive software interface guides users through the experimental setup, data acquisition, and analysis steps, making the system accessible to researchers with varying levels of expertise.
  7. Flexibility: The WES system supports a variety of western blot applications, including protein expression profiling, biomarker discovery, and validation studies, providing versatility for diverse research needs.
  8. Reproducibility: By standardizing the western blotting process and eliminating manual handling steps, the WES system improves assay reproducibility and minimizes inter-assay variability.
Proteins size range 2-440 kDa
Minimum sample size 3uL per capillary
Capillary 5 cm, 100 µm, 400 nL
Maximum Number of Samples 25 (75 samples per day)
Sizing CV <10%
Intra-assay CV <15%
Resolution (± percent difference in MW)

± 15-20% for MW <20 kDA

± 10% for MW >20 kDa

Dynamic Range 2-3 logs (Total Protein Specification)3-4 logs (Immunoassay Specification)
Sensitivity ng(Total Protein Specification)Low pg (Immunoassay Specification)
Process Time 3 hours

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Protein Simple Wes Western Blot System